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Particle Metrix zetaview nta instrument
Zetaview Nta Instrument, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nta+zetaview/zetaview+nta+instrument/pmc11489549__mmc1-8-10-20
Average 90 stars, based on 1 article reviews
zetaview nta instrument - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Concentration Assay:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Staining:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Membrane:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Expressing:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Western Blot:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Fluorescence:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Zymography:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Zeta Potential Analyzer:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).

Transmission Electron Microscopy:

Article Title: Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis.
Article Snippet: For NTA (ZetaView, Particle Metrix), the NEV samples were diluted to 1 mL and injected into the NTA chamber.

Article Title: S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis.
Article Snippet: The particle size, number and distribution of exosomes were determined using NTA (Zetaview, Particle Metrix).

Article Title: Extracellular Vesicles Separation and Biomedical Application Based on Affinity Recognition and Antifouling Coating Bifunctional Microsphere.
Article Snippet: The EVs particle size and concentration were measured through NTA (ZetaView, Particle Metrix, Germany).

Article Title: Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.
Article Snippet: The particle count was determined by NTA (ZetaView, ParticleMetrix).

Article Title: Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles.
Article Snippet: The size, particle concentration of EVs were analyzed by NTA (ZetaView, Particle Metrix, Germany).

Article Title: A Functionalized 3D-Printed Ti6Al4V "Cell Climbing Frame" Inspired by Marine Sponges to Recruit and Rejuvenate Autologous BMSCs in Osteoporotic Bone Repair.
Article Snippet: Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design.. Conventional 3D-printed Ti6Al4V scaffolds are mechanically robust but suffer from poor bone regeneration in osteoporotic patients due to stress shielding and cellular senescence.. In this study, a functionalized 3D-printed Ti6Al4V “Cell Climbing Frame” is developed, aiming to adapt to the mechanical microenvironment of osteoporosis, effectively recruit and support the adhesion and growth of autologous bone marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent cells for improved bone regeneration.

Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity
Article Snippet: The exosomes (100 μl) were diluted to 1 ml to measure the exosome concentration and hydrated diameter and analyzed via NTA (ZetaView, Particle Metrix, Germany).



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90
Particle Metrix nanoparticle tracking analysis (nta) instrument zetaview® twin, pmx-230
Validation of <t>nano</t> particle flow cytometry methodology. A. polystyrene microspheres of the indicated size. B <t>.</t> <t>EVs</t> were enriched from lean (normal chow) or obese (12 weeks high-fat diet) mouse plasma (2µl), with size exclusion chromatography (SEC) and analyzed by flow cytometry. The SSC detector was calibrated to display EV diameter C. CFSE stained EVs from lean and obese mice processed as in B. D. quantification of C E. A representative experiment where plasma is labeled with a cocktail of antibodies for EVs (CD63, CD81, ITGB1), or lipoproteins (APOE, APOB). EVs were enriched with SEC before flow analysis. F. Mouse plasma stained with an adipocyte marker mix (perilipin 1 and adiponectin). Plasma was harvested from either wild type mice or adipocyte-specific PPARγ knockout mice (Adipo-PPARγKO). G . Plasma stained from lean or obese (12 weeks high-fat diet). Total EVs that stain with the adipocyte marker mix (AdipoEVs) and EVs that co-stain with the both the adipocyte and EV marker mixes (Adipo/EV maker). H. Mitochondria isolated from mouse subcutaneous adipose tissue and stained with mitochondrial proteins: VDAC and COXIV or VDAC and TOM20. I. Plasma from mice fed a chow or high-fat diet (6 weeks) stained with markers for the indicated cell types. J. The percentage of EVs from each cell type in mouse plasma. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001
Nanoparticle Tracking Analysis (Nta) Instrument Zetaview® Twin, Pmx 230, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nta+zetaview/zetaview+pmx+220+twin+instrument/pm40523622-87-17-25
Average 90 stars, based on 1 article reviews
nanoparticle tracking analysis (nta) instrument zetaview® twin, pmx-230 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Particle Metrix nanoparticle tracking analysis (nta) instrument zetaview pmx 110
Validation of <t>nano</t> particle flow cytometry methodology. A. polystyrene microspheres of the indicated size. B <t>.</t> <t>EVs</t> were enriched from lean (normal chow) or obese (12 weeks high-fat diet) mouse plasma (2µl), with size exclusion chromatography (SEC) and analyzed by flow cytometry. The SSC detector was calibrated to display EV diameter C. CFSE stained EVs from lean and obese mice processed as in B. D. quantification of C E. A representative experiment where plasma is labeled with a cocktail of antibodies for EVs (CD63, CD81, ITGB1), or lipoproteins (APOE, APOB). EVs were enriched with SEC before flow analysis. F. Mouse plasma stained with an adipocyte marker mix (perilipin 1 and adiponectin). Plasma was harvested from either wild type mice or adipocyte-specific PPARγ knockout mice (Adipo-PPARγKO). G . Plasma stained from lean or obese (12 weeks high-fat diet). Total EVs that stain with the adipocyte marker mix (AdipoEVs) and EVs that co-stain with the both the adipocyte and EV marker mixes (Adipo/EV maker). H. Mitochondria isolated from mouse subcutaneous adipose tissue and stained with mitochondrial proteins: VDAC and COXIV or VDAC and TOM20. I. Plasma from mice fed a chow or high-fat diet (6 weeks) stained with markers for the indicated cell types. J. The percentage of EVs from each cell type in mouse plasma. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001
Nanoparticle Tracking Analysis (Nta) Instrument Zetaview Pmx 110, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nta+zetaview/nanoparticle+tracking+analysis+zetaview/pm40514650-79-12-16
Average 90 stars, based on 1 article reviews
nanoparticle tracking analysis (nta) instrument zetaview pmx 110 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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Validation of nano particle flow cytometry methodology. A. polystyrene microspheres of the indicated size. B . EVs were enriched from lean (normal chow) or obese (12 weeks high-fat diet) mouse plasma (2µl), with size exclusion chromatography (SEC) and analyzed by flow cytometry. The SSC detector was calibrated to display EV diameter C. CFSE stained EVs from lean and obese mice processed as in B. D. quantification of C E. A representative experiment where plasma is labeled with a cocktail of antibodies for EVs (CD63, CD81, ITGB1), or lipoproteins (APOE, APOB). EVs were enriched with SEC before flow analysis. F. Mouse plasma stained with an adipocyte marker mix (perilipin 1 and adiponectin). Plasma was harvested from either wild type mice or adipocyte-specific PPARγ knockout mice (Adipo-PPARγKO). G . Plasma stained from lean or obese (12 weeks high-fat diet). Total EVs that stain with the adipocyte marker mix (AdipoEVs) and EVs that co-stain with the both the adipocyte and EV marker mixes (Adipo/EV maker). H. Mitochondria isolated from mouse subcutaneous adipose tissue and stained with mitochondrial proteins: VDAC and COXIV or VDAC and TOM20. I. Plasma from mice fed a chow or high-fat diet (6 weeks) stained with markers for the indicated cell types. J. The percentage of EVs from each cell type in mouse plasma. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001

Journal: bioRxiv

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts

doi: 10.1101/2025.07.11.664236

Figure Lengend Snippet: Validation of nano particle flow cytometry methodology. A. polystyrene microspheres of the indicated size. B . EVs were enriched from lean (normal chow) or obese (12 weeks high-fat diet) mouse plasma (2µl), with size exclusion chromatography (SEC) and analyzed by flow cytometry. The SSC detector was calibrated to display EV diameter C. CFSE stained EVs from lean and obese mice processed as in B. D. quantification of C E. A representative experiment where plasma is labeled with a cocktail of antibodies for EVs (CD63, CD81, ITGB1), or lipoproteins (APOE, APOB). EVs were enriched with SEC before flow analysis. F. Mouse plasma stained with an adipocyte marker mix (perilipin 1 and adiponectin). Plasma was harvested from either wild type mice or adipocyte-specific PPARγ knockout mice (Adipo-PPARγKO). G . Plasma stained from lean or obese (12 weeks high-fat diet). Total EVs that stain with the adipocyte marker mix (AdipoEVs) and EVs that co-stain with the both the adipocyte and EV marker mixes (Adipo/EV maker). H. Mitochondria isolated from mouse subcutaneous adipose tissue and stained with mitochondrial proteins: VDAC and COXIV or VDAC and TOM20. I. Plasma from mice fed a chow or high-fat diet (6 weeks) stained with markers for the indicated cell types. J. The percentage of EVs from each cell type in mouse plasma. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001

Article Snippet: Samples were incubated at 37°C for 30 minutes and EVs purified by SEC. EVs were counted by nano particle tracking analysis (NTA; ZetaView by Particle Metrix) and 1×10 8 labeled EVs per gram body weight (∼2×10 9 EVs per 20g mouse) were injected.

Techniques: Biomarker Discovery, Flow Cytometry, Clinical Proteomics, Size-exclusion Chromatography, Staining, Labeling, Marker, Knock-Out, Isolation

Circulating adipocyte EVs and EV-mitos are increased with high-fat feeding early and persistently. A. Body weights for a cohort of WT male and female (N=6 for each sex and group). B. Nano particle flow cytometry for lipoprotein markers in mice plasma (APOB/E antibodies). Mice were on diets for 6 weeks. C-D Total EVs in plasma (CFSE labeled) were quantified at day 0 and day 1 of the feeding experiment ( C ) or at baseline, and 30 or 60 minutes after bleeding ( D ). Plasma as collected from male and female cohorts (N=6) via tail vein bleed over the indicated time course after diet initiation (chow or high-fat diet; HFD). E-L EVs were analyzed for total EVs (CFSE-stained; E-F ), adipocyte EVs (adipoEVs; APN/PLN1 + ; G-H ), adipocyte EV-mitos (adipoEV-mitos; APN/PLN1 + , VDAC + ; I-J ), and total mitochondria (VDAC + , K-L ). E, G, I and K are acute timepoints. F, H, J, L are chronic timepoints. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001.

Journal: bioRxiv

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts

doi: 10.1101/2025.07.11.664236

Figure Lengend Snippet: Circulating adipocyte EVs and EV-mitos are increased with high-fat feeding early and persistently. A. Body weights for a cohort of WT male and female (N=6 for each sex and group). B. Nano particle flow cytometry for lipoprotein markers in mice plasma (APOB/E antibodies). Mice were on diets for 6 weeks. C-D Total EVs in plasma (CFSE labeled) were quantified at day 0 and day 1 of the feeding experiment ( C ) or at baseline, and 30 or 60 minutes after bleeding ( D ). Plasma as collected from male and female cohorts (N=6) via tail vein bleed over the indicated time course after diet initiation (chow or high-fat diet; HFD). E-L EVs were analyzed for total EVs (CFSE-stained; E-F ), adipocyte EVs (adipoEVs; APN/PLN1 + ; G-H ), adipocyte EV-mitos (adipoEV-mitos; APN/PLN1 + , VDAC + ; I-J ), and total mitochondria (VDAC + , K-L ). E, G, I and K are acute timepoints. F, H, J, L are chronic timepoints. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001.

Article Snippet: Samples were incubated at 37°C for 30 minutes and EVs purified by SEC. EVs were counted by nano particle tracking analysis (NTA; ZetaView by Particle Metrix) and 1×10 8 labeled EVs per gram body weight (∼2×10 9 EVs per 20g mouse) were injected.

Techniques: Flow Cytometry, Clinical Proteomics, Labeling, Staining

AdipoEVs correlate with insulin resistance in people with obesity. Plasma samples from metabolically healthy lean (MHL), metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) patients were analyzed by nano flow cytometry for A. Lipoproteins (APOE/B + ), B. total EVs (CFSE + ), C. adipoEVs (APN/PLN1 + ), D. adipoEV-mitos (APN/PLN1 + , VDAC + ), E. total mitochondria (VDAC + ), F. Adipose tissue (AT) immune cell EVs (APN/PLN + , CD45 + ), G. total immune cell EVs (CD45 + ), or H. Endothelial cell (EC) EVs (CD31 + /CD45 - ). AdipoEVs correlations with I. % fat mass J. insulin sensitivity, K. hepatic insulin sensitivity index (HISI), L. homeostatic model assessment of insulin resistance (HOMA-IR), M. Intrahepatic triglyceride (IHTC) content. Data are presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: bioRxiv

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts

doi: 10.1101/2025.07.11.664236

Figure Lengend Snippet: AdipoEVs correlate with insulin resistance in people with obesity. Plasma samples from metabolically healthy lean (MHL), metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) patients were analyzed by nano flow cytometry for A. Lipoproteins (APOE/B + ), B. total EVs (CFSE + ), C. adipoEVs (APN/PLN1 + ), D. adipoEV-mitos (APN/PLN1 + , VDAC + ), E. total mitochondria (VDAC + ), F. Adipose tissue (AT) immune cell EVs (APN/PLN + , CD45 + ), G. total immune cell EVs (CD45 + ), or H. Endothelial cell (EC) EVs (CD31 + /CD45 - ). AdipoEVs correlations with I. % fat mass J. insulin sensitivity, K. hepatic insulin sensitivity index (HISI), L. homeostatic model assessment of insulin resistance (HOMA-IR), M. Intrahepatic triglyceride (IHTC) content. Data are presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Samples were incubated at 37°C for 30 minutes and EVs purified by SEC. EVs were counted by nano particle tracking analysis (NTA; ZetaView by Particle Metrix) and 1×10 8 labeled EVs per gram body weight (∼2×10 9 EVs per 20g mouse) were injected.

Techniques: Clinical Proteomics, Metabolic Labelling, Flow Cytometry